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Expression of vesicular glutamate transporter in transient receptor potential vanilloid 1-positive neurons in the rat trigeminal ganglion

International Journal of Oral Biology 2021³â 46±Ç 3È£ p.119 ~ 126
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ÇÑÇý¹Î ( Han Hye-Min ) - Kyungpook National University School of Dentistry Department of Anatomy and Neurobiology
Á¶À̽½ ( Cho Yi-Sul ) - Kyungpook National University School of Dentistry Department of Anatomy and Neurobiology
¹è¿ëö ( Bae Yong-Chul ) - Kyungpook National University School of Dentistry Department of Anatomy and Neurobiology

Abstract


Activation of transient receptor potential vanilloid 1 (TRPV1), a calcium permeable channel expressed in primary sensory neurons, induces the release of glutamate from their central and peripheral afferents during normal acute and pathological pain. However, little information is available regarding the glutamate release mechanism associated with TRPV1 activation in primary sensory neurons. To address this issue, we investigated the expression of vesicular glutamate transporter (VGLUT) in TRPV1-immunopositive (+) neurons in the rat trigeminal ganglion (TG) under normal and complete Freund¡¯s adjuvant (CFA)-induced inflammatory pain conditions using behavioral testing as well as double immunofluorescence staining with antisera against TRPV1 and VGLUT1 or VGLUT2. TRPV1 was primarily expressed in small and medium-sized TG neurons. TRPV1+ neurons constituted approximately 27% of all TG neurons. Among all TRPV1+ neurons, the proportion of TRPV1+ neurons coexpressing VGLUT1 (VGLUT1+/ TRPV1+ neurons) and VGLUT2 (VGLUT2+/TRPV1+ neurons) was 0.4% ¡¾ 0.2% and 22.4% ¡¾ 2.8%, respectively. The proportion of TRPV1+ and VGLUT2+ neurons was higher in the CFA group than in the control group (TRPV1+ neurons: 31.5% ¡¾ 2.5% vs. 26.5% ¡¾ 1.2%, VGLUT2+ neurons: 31.8% ¡¾ 1.1% vs. 24.6% ¡¾ 1.5%, p < 0.05), whereas the proportion of VGLUT1+, VGLUT1+/TRPV1+, and VGLUT2+/TRPV1+ neurons did not differ significantly between the CFA and control groups. These findings together suggest that VGLUT2, a major isoform of VGLUTs, is involved in TRPV1 activation-associated glutamate release during normal acute and inflammatory pain.

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Transient receptor potential vanilloid 1; Vesicular glutamate transporter; Glutamate; Pain; Trigeminal ganglion

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